Droplet-train Spr Microchip for Label-free Detection of Bio-interaction Using Nanoliters of Drug Sample
نویسندگان
چکیده
We demonstrate a new Droplet-Train SPR technique for label-free detection and quantification of bio-interactions on fixed volumes of samples. Bio-samples are first discretized within ~100 nL plug droplets separated by an immiscible phase. The plugs are sequentially transported onto SPR sensing sites within a surface-modified PDMS microchip. The measured kinetic constants ka and kd for the model protein-drug interaction are 8.33×10 3 1/M-s and 4.58×10 -2 1/s, matching well with previous reports.
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تاریخ انتشار 2012